Zetasizer Nano – 10 Analytical Benefits

The Malvern Panalytical Zetasizer Nano is highly popular and is found in a wide range of basic and applied scientific projects across all fields and industries. It is the most broad-ranged and dynamic instrument in the market for solving technical issues in today's research environment and it continues to evolve with new capabilities.

New measurement techniques are being introduced for the Zetasizer Nano including a surface zeta potential accessory and a new diffusion barrier method for improved zeta potential measurement of protein samples.

Ten top reasons why one should choose the Zetasizer Nano are listed below.

Zeta Potential Measurement with Surface Zeta Potential Cell

It is possible to obtain zeta potential measurement of a solid surface with a newly designed cell for the Zetasizer Nano Instrument. This will enable researchers to study the material surface and desorption or adsorption process from that surface.

The results will offer information on coating surfaces and the interface of a material. Hence it is possible to change the chemistry of a material substrate and obtain results on the surface activity of surfactants or other formulation materials onto a solid surface.

Measuring Protein Samples with New Diffusion

This measurement technique will make use of the standard disposable/reusable capillary zeta potential cells. A cell is filled with the same buffer used as the dispersing medium for the protein sample to be measured. A small volume (50pL) of protein is then injected into the bottom of the cell as a plug of sample.

This offers a protein plug that is far from the electrode surface of the zeta potential cell. This technique is known as a diffusion barrier technique and it protects the protein sample from denaturation by introducing a physical distance between the sample and the electrodes

Use of the High Concentration Low Volume Cell

Irrespective of the sample volume or if the sample is a concentrated opaque sample, the high concentration, low volume cell can be used. A high concentration zeta potential cell option that makes use of palladium electrodes and a very small capillary enables sample measurement at higher concentrations than possible previously.

Two highly desired sample cells are combined in this sample cell and zeta potential measurements can be made on samples up to 40wt% solids while requiring low sample volumes (as little as 150pL of sample.)

Electophoretic Mobility of Non-Aqueous Samples

The Universal Dip Cell accessory is used to measure the electrophoretic mobility of non-aqueous samples. The universal dip cell is resistant to samples and can be used for non-aqueous and aqueous samples.

Malvern Panalytical’s Patented M3-PALS Method

Zeta Potential Measurements are carried out using Malvern Panalytical's patented M3-PALS method. Zeta potential results with the M3-PALS method offer highest accuracy and sensitivity in the market.

The mixed mode measurement by Malvern Panalytical was patented and invented for excellent accuracy and ease of use.

Dynamic Light Scattering Method for Size Measurement

The Zetasizer Nano can offer sample size measurement in the range of 0.3nm to 10µm in diameter. It can also measure samples that are very dilute - 0.1ppm to very high concentration in the order of 40wt% solids, based on the sample type. Its detection system is self-optimizing and the software adjusts the optics, attenuation and measurement duration for producing reliable and consistent data.

Molecular Weight Characterization

Using static light scattering and the Debye method, molecular weight characterization is available by adding the Zetasizer Nano to one’s size exclusion chromatography system. Static light scattering information can be obtained from DLS instrumentation. SLS results offer weight average molecular weight using the Debye method.

It is also possible to add the Zetasizer Nano as an additional detector to an SEC system to provide MW information both by estimating MW from absolute size measurements, or combined with a concentration signal to give absolute molecular weight.

Non-Invasive Back-Scatter Technology

Non-invasive back scatter technology enables the use of a wide range of low volume, disposable glass or quartz sample cells. The broad array of sample cuvettes offer researchers flexibility in their analysis protocols and this flexibility enables improved data quality, quicker results and higher assurance of maintaining sample purity.

Integration of an Auto-Titrator

It is also possible to integrate an auto titrator to automate conductivity and pH titrations as well as additive and dilution measurements. The measurement of size or zeta potential as a function of pH, conductivity or additive concentration can offer insights into the processes involved in flocculating or stabilizing dispersed systems.

Ease of Use and Software Flexibility

Finally, the software flexibility and ease of use is not like any other scientific instrument in the market. The Zetasizer software is suited for a multi-user environment. It is possible for even a summer student or a visitor to use this system very quickly and easily.

About Malvern Panalytical

Malvern Panalytical provides the materials and biophysical characterization technology and expertise that enables scientists and engineers to investigate, understand and control the properties of dispersed systems. These systems range from proteins and polymers in solution, particle and nanoparticle suspensions and emulsions, through to sprays and aerosols, industrial bulk powders and high concentration slurries. Used at all stages of research, development and manufacturing, Malvern Panalytical’s instruments provide critical information that helps accelerate research and product development, enhance and maintain product quality and optimize process efficiency.

This information has been sourced, reviewed and adapted from materials provided by Malvern Panalytical.

For more information on this source, please visit Malvern Panalytical.

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