In a new paper published in Polymers & Polymer Composites (Vol 21, No 1, 2013), researchers at Kingston University in the UK report the results of continuing work to examine the effects of gamma irradiation on intraocular lenses (IOLs) used in cataract surgery. Triple detection gel permeation chromatography (GPC) using the Viscotek TDAmax system from Malvern Panalytical was a key analytical tool in this work.
(from left to right) Dr Baljit Ghatora (Lecturer in Forensic and Analytical Chemistry), Prof Peter Foot (Professor in Materials Chemistry), Dr Stephen Barton (Principal Lecturer in Analytical Chemistry), Kingston University.
Baljit Ghatora and co-authors examined medical grade poly(methyl methacrylate) (PMMA), which is used especially widely in developing countries to make rigid intraocular lenses and for which low cost, convenient gamma irradiation is increasingly the preferred method for sterilisation. Previous reports have suggested that gamma irradiation has the potential to alter the structural features of the polymer, such as molecular weight and molecular size distribution, which in turn may impact the efficacy and performance of IOLs.
The newly published study follows earlier work that examined whether cross linking or chain scission predominated in the degradation process. It reports on comparisons of structural differences between gamma-irradiated and control medical-grade PMMA discs analyzed using triple detection GPC, as well as examining fluid uptake by the irradiated lenses following immersion in simulated aqueous humour solution (SAHS) and post-irradiation plasticisation determined using thermomechanical analysis.
Malvern Panalytical’s triple detection GPC, combining size exclusion chromatography with light scattering measurements and viscometry, was applied specifically to determine absolute molecular weights and molecular weight distribution of the gamma sterilised PMMA, allowing these to be compared directly with control values.
The paper contributes to an increasing body of literature on how manufacturing processes can affect the usage of PMMA IOLS in cataract surgery.
For further details of the work taking place in the Materials Research Centre at Kingston University London, go to http://sec.kingston.ac.uk/about-SEC/people/academic/view_profile.php?id=130
For information about Malvern Panalytical’s triple detection GPC visit www.malvern.com/viscotek