Prep TLC, also known as Preparative TLC, is utilized on a bigger scale than regular TLC to purify and isolate analytes from contaminants.
Prep TLC is a quick, low-cost, and effective process for purifying tiny amounts of material. It enables the fast separation, isolation, and recovery of components in a reaction mixture.
Different layer thicknesses (500 µm, 1000 µm, and 2000 µm) are offered for preparative reasons.
A slightly coarser material is employed for 2000 µm preparative layers.
The isolated spots can be scraped from a prep plate, collected, and used for additional downstream analysis.
Prep TLC Features
- Rapid and cost-effective identification/isolation of compounds from a reaction
- Thicker layers than classical TLC for higher sample loading volumes
- Separation and purification from milligram to gram quantities
- Offline qualitative evaluation of purity during a process
Image Credit: Sorbent Technologies, Inc.
Prep TLC Loading and Resolution Guide
Image Credit: Sorbent Technologies, Inc.