The FLUOstar® Omega is an ideal plate reader for all life science applications. It is a multi-mode microplate reader, with six detection modes. For absorbance, it uses an ultra-fast UV/vis spectrometer or filters, and highly sensitive filters for all other detection modes.
Product Details
The six detection modes used in the versatile FLUOstar® Omega multi-mode plate reader are: fluorescence intensity (including FRET), luminescence (including BRET), UV/vis absorbance, time-resolved fluorescence, TR-FRET, and AlphaScreen®/AlphaLISA®.
Features
- High-speed absorbance measurements
- Measurements in up to 384-well plates
- Top and bottom reading
- Incubation up to 45 or 65 °C
- User-defined shaking
- Well scanning with up to 900 data points per well
- High-precision injectors with simultaneous reagent injection and detection
- Low-volume measurements
Accessories
- Atmospheric Control Unit (ACU): provides the ideal environment for cell-based assays by regulating O2 and CO2 gas levels in the microplate reader
- Filters and Optic Modules: standard filters from 240 - 850 nm, filters and Optic Modules optimised for specific applications, and bespoke solutions are available
- Microplate Stacker: optimises microplate handling and increases throughput by providing rapid loading, unloading, restacking, and continuous feeding for up to 50 microplates
- LVis Plate: microplate for low-volume DNA, RNA and protein quantification. It includes 16 micro-drop (2 µL) wells, a cuvette slot and optional performance testing features
- Evaluation Plate: validates the optical functionality of BMG LABTECH microplate readers for quality control and/or qualification (IQ/OQ) purposes
Technical Specifications
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Detection modes
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Fluorescence intensity - incl. FRET
AlphaScreen®/AlphaLISA®
Luminescence (flash and glow) - incl. BRET
Time-resolved fluorescence
TR-FRET
UV/Vis absorbance spectra
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Measurement modes
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Top and bottom reading
Endpoint and kinetic
Sequential multi excitation
Sequential multi emission
Spectral scanning (absorbance)
Ratiometric measurements
Well scanning
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Microplate formats
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6 to 384-well plates, user-definable
LVis Plate with 16 low volume microspots (2 μL)
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Microplate carrier
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Robot compatible
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Light sources
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High energy xenon flash lamp
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Detectors
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Low noise photomultiplier tube
CCD spectrometer
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Wavelength selection
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Optical filters: ex and em wheels for 8 filters each
UV/vis absorbance spectrometer: full spectra or 8 distinct wavelengths in < 1 s/well
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Optical filters
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Excitation and emission filter wheels for 8 filters each
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Optical path guides
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Top: liquid-filled light guides
Bottom: fiber optics
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Spectral range
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Filters: 240–740 nm or 240–900 nm for FI, FP, TRF
240–740 nm for LUM
Spectrometer: 220–1000 nm for ABS
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Sensitivity
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FI
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< 0.2 fmol/well FITC
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TRF
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< 30 amol/well europium
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High-End TRF for Omega
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< 3 amol/well europium
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LUM
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< 20 amol/well ATP
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AlphaScreen®
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< 5 pM (< 100 amol/well P-Tyr-100, 384 sv, 20 μL)
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ABS
with
spectrometer
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Full spectrum captured in < 1 s/well
Selectable spectral resolution: 1, 2, 5 and 10 nm
OD range: 0 to 4 OD
Accuracy: < 1% at 2 OD
Precision: < 0.5% at 1 OD and < 0.8% at 2 OD
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Read times
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Flying mode (1 flash), 9 s (96), 16 s (384)
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Reagent injection
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Up to 2 built-in reagent injectors
Injection at measurement position (6 to 384-well)
Individual injection volumes for each well (3 to 500 µL)
Variable injection speed up to 420 µL/s
Up to four injection events per well
Reagent back flushing
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Shaking
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Linear, orbital, and double-orbital with user-definable time and speed
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Purge gas vent
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System to inject an atmosphere or to pull a vacuum into the reader
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Incubation
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+4 °C above ambient up to 45 °C or 65 °C
The upper heating plate of the incubation chamber operates at 0.5 °C more than the lower plate. This prevents condensation build-up on the lid or sealer.
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Software
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Multi-user Reader Control and MARS Data Analysis Software included
FDA 21 CFR Part 11 compliant
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Dimensions
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Width: 44 cm, depth: 48 cm, height: 30 cm; weight: 28 kg
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Optional Accessories
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LVis plate
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Sample capacity: 16 separate microdrop wells for 2 µL samples; one standard cuvette position for up to 1 mL samples.
Quality control internal standards (optional): four NIST traceable optical density filters (approximate values of 0.1, 0.3, 0.6 and 1.0 OD); one holmium oxide filter for wavelength accuracy
Dimensions: conforms to SBS standards for microplates.
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Stacker
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Magazines for up to 50 plates - continuous loading feature
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THERMOstar
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Microplate incubator and shaker
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Optical filters
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Excitation and emission filters
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Upgrades
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Please contact your local representative for upgrades including options such as detection modes, reagent injectors, etc.
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LOD = 3x SD (20 blanks)/slope (6 pt std curve)
AlphaScreen® P-Tyr-100 assay kit, PerkinElmer, #6760620C
Microplates: white for LUM, AlphaScreen®, TRF; black for FI, FP; clear for ABS
96 = 96-well microplates
384 sv = 384-well small volume microplates
384 g = 384-well glass bottom microplates
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Studying the process of fertilisation on BMG LABTECH readers