The Peptide-Builder is designed for peptide chemists who want to make high-quality peptides quickly and easily at a laboratory scale. Peptides are synthesized sequentially and fully automated either one at a time or queued up to 16, for example, overnight.
- Synthesis scale between 0.05 and 1.0 mmol
- Uses Vapourtec’s patented reactor technology for continuous flow single pass SPPS
- Fmoc solid phase peptide chemistry with DIC and oxyma activation
- Completely automated for up to 16 peptides
- Constant in-line analytics, such as UV absorption and resin solvation data
- Quick cycle times
- Automatically adding a side chain or chains as needed
Peptide Synthesizer - The Peptide Builder
Video Credit: VAPOURTEC Ltd
Synthesis Scale 0.05 – 1.00 mmol
The Peptide Builder accommodates a wide range of synthesis scales, from 50 µmol to 1.0 mmol. It can be used with any kind of polymeric resin, including PS, PEGylated resins, and the standard assortment of linkers.
Resins are loaded into barcoded resin cartridges before being put into the resin cartridge carousel for automated processing. The barcode guarantees that the finished peptide is correctly identified.
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Fully Automated up to 16 Peptides
Users can program 16 distinct peptide sequences at any time by utilizing the simplicity of a carousel.
Since these syntheses will be carried out sequentially, the user will have easy access to remove the desired peptide from the system if it is the first in line. The peptide will be synthesized in a matter of hours.
The interface of the cutting-edge GMP software makes it simple to program the synthesis of sixteen distinct peptides. The system will unload the resin cartridge and prepare it for cleavage upon completion of the synthesis.
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Uses Vapourtec’s Patented Reactor Technology (VBFR) for Continuous Flow Single Pass SPPS
Vapourtec created and patented a novel reactor technology for synthesizing peptides in 2017. This reactor is called the Variable Bed Flow Reactor (VBFR).
This reactor's main advantage is keeping the resin's packing density constant throughout the synthesis process. The packaging density is maintained as the resin's volume changes during synthesis, guaranteeing the best possible interaction between the peptidyl resin and the reagents and solvents. The reactor volume is reduced and channeling is removed.
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Heated Reactors (VBFR) Ensure Fast Cycle Times
The variable bed flow reactor (VBFR) technology, patented by Vapourtec, has accurate temperature control.
Heating has two major advantages: it can increase reaction kinetics, which can be especially helpful for sterically challenging couplings, and it can prevent β-sheet structures, which can cause aggregation events.
With DIC and Oxyma as activating reagents, a 7-minute cycle time is attained. A typical 30-mer can be synthesized in less than 3.5 hours even when side chains are added.
Rapid temperature changes for reactions enable the selection of the ideal temperature for every amino acid. Under the right circumstances, residues prone to racemization can be coupled and activated. The state-of-the-art Peptide-Builder software automatically chooses the best reactor temperatures for each of the natural amino acids.
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Minimizes Solvent and Reagent Usage
Solvent usage is generally under 70 ml per mmol per cycle, even when using PEG resins.
Although 1.5–3 equivalents of amino acids are used in typical syntheses, costly unnatural amino acids can be used as the limiting reagent if desired due to the single-pass continuous flow.
For instance, less than 70 mm of solvent is needed to synthesize a 10-mer peptide at a 0.1 mmol scale.
Ten liters of DMF can be stored in the Peptide-Builder, which also has a level indicator that will halt synthesis if the reservoir runs out.
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Continuous In-Line Analytics, Including Resin Solvation Data and UV Absorption Data
A major advantage of VBFR technology is the ability to measure reactor volume in real-time. This yields entirely new information about the synthesis of peptides, offering fresh perspectives on peptide aggregation. Each aggregation event's exact timing and size can be tracked and measured in real-time.
Based on this information, new and more rigorous scientific research can be done using pseudo-proline dipeptides or other aggregation mitigation strategies.
The single-pass continuous flow makes it easier to precisely quantify the Fmoc deprotection from the resin in addition to the resin solvation data. The most advanced GMP software computes, plots, and displays the area under the curve (AUC) for every deprotection in real-time.
The GMP software will email the user the data as a detailed report and a graphical chart after the synthesis is finished.
Image Credit: VAPOURTEC Ltd