Stunner from Unchained Labs is the only system that pulls together Dynamic Light Scattering (DLS), Static Light Scattering (SLS), and UV/Vis concentration data from the same 2 μL sample.
Users can dig into the adenovirus vector (AdV) or AAV to get the total capsid titer and empty/full ratio or rack up payload concentration and size data on any nanoparticle simultaneously.
Nail down the quality of protein by knocking hydrodynamic size, concentration, polydispersity, and detection of aggregates off the list all at once. Users will know whether AdV, AAV, nanoparticle, or protein is good to go without skipping a beat.
- AAV & AdV empty/full ratio
- AAV & AdV capsid titer
- LNP total RNA quant
- Aggregation
- Concentration
- Nanoparticle payload quant
- Sizing & polydispersity
- B22 & kD
Small Sample, Lots of Information
In a Stunner plate, 2 μL of sample can be loaded without sample prep or dilution.
To cover a broad dynamic range of 0.03-275 OD, all microfluidic circuits have two fixed pathlengths built into them.
If users are dealing with a full plate, they can get 96 concentration measurements in 10 minutes—DLS sizing can be added and completed in one hour.
Users can hook it up to their favorite robot to add more potency for even heavier workflows.
Image Credit: Unchained Labs
Users can conveniently input their AAV, and SLS and DLS can swiftly determine the count of intact capsids or detect any interference from aggregated clusters.
In one minute, they can observe the total protein content, assess the empty/full ratio, and measure the total ssDNA using UV/Vis. Users do not need to worry about spectral overlap or extinction coefficient calculations, as Stunner handles all these computations for you.
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Know AAV Inside Out
Users can quickly obtain the specific titers they require with Stunner. This powerful tool integrates UV/Vis and DLS data to accurately quantify the number of empty and full capsids and the remaining excess DNA and protein.
It enables users to achieve titers as low as 10^12 vg/mL with purified AAV. In a single assay, Stunner's dye-free, label-free, standard-free, and hassle-free workflow tells the complete titer story.
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Titer for Any Serotype
All AAVs are unique; however, Stunner figures them all out. Users can pick a preloaded serotype or feed in the specs for the virus and get answers in seconds. Users can check capsid titers and empty/full ratio as frequently as they like.
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Aggregates Ruin Everything
Globs of AdV or AAV can stand between the user and high-quality information for all assays. DLS checks if the AdV or AAV is monodisperse so the remaining process is not tripped up through aggregates.
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With Stunner’s readouts on full, total, and empty capsids, users can get legit adenovirus (AdV) titers down to 109 cp/mL without getting thrown off by frustrating contaminants such as extra DNA and aggregates.
Conventional A260 measurements are outsmarted by Unmixing the absorbance of AdV into protein and dsDNA signal—so that users get the low down on what is actually in the sample.
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See Through the Fog
Turbid solutions of LNPs and various nanoparticles can present challenges for conventional methods, but Stunner's combination of short pathlengths along with DLS and UV/Vis can provide the solutions users are seeking. By utilizing Unmix, users can efficiently bypass the turbidity and isolate the absorbance signal from payloads, offering clear insights.
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Break Down Data, Not Particles
Users can teach Stunner about the UV/Vis absorbance of the nanoparticle, which will accurately detect the signal. They can skip the disruption step and measure any payload: DNA, RNA, protein, or small molecule.
Stunner will make quantification simple to free users from complex disruption workflows, wasteful standard curves, and costly dyes.
Image Credit: Unchained Labs
Slam Through Tons of Nanoparticle Sizing
In less than one hour, the DLS gives the high-throughput power to round up size and size distribution data on 96 nanoparticle samples.
Users can build up sizing statistics using as many replicates as they desire and reduce the time at the bench.
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Max Out Biologic
Stunner is the only system that can quantify biologics at high concentration and high throughput. It comes with a dynamic range spanning between 0.02 mg/mL and 200 mg/mL (mAb)—so that users can run any protein without having to dilute ever again. Users can run more than one protein at a time without cleaning up.
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Get the Skinny on Proteins
Stunner outperforms other systems by offering a comprehensive range of capabilities beyond DLS. It empowers users to understand how factors like storage conditions, agitation, or modifications to process/formulation impact proteins. Users can identify potential aggregation, assess hydrodynamic size, evaluate uniformity through polydispersity measurements, and precisely determine concentration—all in one versatile platform.
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Precise Data
Get spot-on accuracy within 1% and 2%. With two fixed pathlengths, Stunner gives precise data at both low and high protein concentrations and always fixes the required concentrations. Stunner is the instrument for protein quantification with the tiniest sample size, outstanding precise results, and the highest throughput.
Image Credit: Unchained Labs
Source: Unchained Labs
Brew Their Own
Users can find out what else is going on with the sample, pick a background subtraction method and a few wavelengths, and create their favorite equation.
The homebrew tool is wide open so that users can make apps just the way they prefer them.
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Double Down with B22 and kD
On the fly data tells users if the sample is in good condition or if aggregation is needed.
As the concentration of all samples in the series is quantified in real-time using DLS, users get the most precise values with no extra effort.
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Accuracy Users Can Show Off
Using the Fundamentals tryptophan standards, the instrument's precision, accuracy, and linearity can be established at any point at the OD range of 20-225 and protein-relevant 280 nm wavelength. Users can be confident about all samples without any doubt.
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Downstream Ready
Users can quickly go through the USP & Ph. Eur. UV/Vis standards for absorbance precision, accuracy, linearity, stray light, wavelength accuracy, and resolution with performance verification measurements of independently certified NIST standards.
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Put it on Lockdown
Stunner hooks labs up with 21 CFR Part 11 compliant characteristics, which include electronic signatures, password protection, and a full audit trail.
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