Posted in | News | Plastics and Polymers

New Application Note on Light Scattering/Gel Permeation Chromatography from ViscoTek

Oct 2 2008

Viscotek has announced an on-demand presentation (http://www.viscotek.com/gpcsels.aspx) and application note that describes a new light scattering technique that could be very helpful in Gel Permeation Chromatography (GPC) applications where the demands of sample solvent and mobile phase are different.

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Solvent enhanced light scattering (SELS) in combination with Gel Permeation Chromatography (GPC) allows users-with two different solvents-to separately optimise the sample preparation step and the chromatographic conditions to increase the dn/dc value to get better light scattering responses.

In the application note an example of a fluoropolymer analysis by SELS-GPC is shown. Although the fluoropolymer is quite soluble in Tetrahydofuran (THF) the dn/dc in this solvent is very low and the light scattering response poor. Whilst using a low refractive index solvent like Acetone increases dn/dc the sample is not soluble in Acetone. But by dissolving the sample in THF and injecting the solution into a mobile phase of Acetone (SELS concept) the researchers were able to maximise the accuracy and reliability of the GPC-LS analysis while using a cheap and safe solvent as mobile phase.

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