Talos L120C G2 (S)TEM - Versatile 2D and 3D Visualization for Beam-Sensitive Samples

The Thermo Scientific Talos L120C G2 (S)TEM is a 20–120 kV thermionic (scanning) transmission electron microscope with a unique design for efficiency and productivity. It works well with a variety of samples and applications, including soft materials, asbestos, polymers, and cells in both 2D and 3D, at both room temperature and cryogenic temperatures.

Users can acquire excellent results with the Talos L120C with little effort. The user interface (UI) for routine 2D imaging is straightforward and user-friendly, simplifying the imaging process.

This interface, combined with rapid and advanced automation in 3D imaging workflows, enhances TEM and STEM resolution for 20-120 kV instruments. This design enables users to concentrate more on scientific questions and less on the specifics of how to operate the microscope.

Explore cellular ultrastructure. Image Credit: Thermo Fisher Scientific – Electron Microscopy Solutions

Gain insights into elemental composition. Image Credit: Thermo Fisher Scientific – Electron Microscopy Solutions

Characterize quality attributes of nanoparticles. Image Credit: Thermo Fisher Scientific – Electron Microscopy Solutions

Visualize 3D intracellular structures. Image Credit: Thermo Scientific - Materials and Structural Analysis

Talos L120C (S)TEM) Advantages

The Talos L120C elevates imaging capabilities with its optional cryo-box and low-dose methods. These features enable the imaging of samples maintained at cryogenic temperatures and yield high-quality images, particularly for materials sensitive to beam exposure. The cryo-box offers optimal protection for cryo-specimens, effectively minimizing ice growth contamination during extended (>8 hours) data collection sessions.

To boost both productivity and versatility in environments with multiple users and applications, the cryo-box of the Talos L120C is designed to be motorized and fully retractable. This feature enables seamless transitioning between cryo-EM imaging, STEM imaging, and EDS analysis.

Additionally, the C-Twin lens's large pole-piece gap ensures high contrast and accommodates specimen tilts of up to 90°. The constant-power objective lens, characterized by low-hysteresis, facilitates quick and reproducible changes in imaging modes and high-tension settings in a matter of seconds.

Overall, the Talos L120C (S)TEM combines application versatility with a reproducibly performing electron column to give users new opportunities for high-resolution 3D characterization, in situ dynamic observation with EDS and other diffraction applications, and a special emphasis on high-contrast imaging and cryo-TEM.

Talos L120C (S)TEM Automation

Specifically designed for multi-user and multi-discipline environments and equipped with a familiar UI shared across all Thermo Scientific TEM platforms, the Talos L120C TEM is the perfect solution for both expert and novice users.

All daily TEM tunings have been fully automated to ensure the best and most reproducible setup possible. This automation simplifies the learning process for new operators and enhances the efficiency of data acquisition for experienced users. 

The Talos L120C TEM also features an online educational help platform embedded within the UI. Thermo Scientific application software packages also facilitate intuitive settings and automated data collection for different use cases and workflows, such as large area 2D imaging, energy dispersive X-Ray analysis, single particle analysis, MicroED, and tomography.

Talos L120C (S)TEM Key Features

Versatile Multi-Application System

Designed for routine TEM and STEM imaging and characterization of a range of samples at cryogenic and ambient temperatures, including soft materials, protein complexes, resin-embedded cells, and nanoparticles.

Cryo-Imaging

Superior imaging of samples at cryogenic temperatures is made possible by a retractable, motorized cryo-box and a low-dose technique, which also preserves the possibility of STEM imaging and EDS analysis applications.

Space for More

Add tomography or in situ sample holders compatible with a large analytical pole piece gap, allowing up to ±90^° stage tilt range and ±375 µm stage height range.

Auto-Alignments

All daily TEM tunings—such as focus, Eucentric height, center beam shift, center condenser aperture, and rotation center—are automated.

Ceta CMOS Camera

The 4K × 4K Thermo Scientific Ceta CMOS Camera features a large field of view, high sensitivity, and speed across all high-tension ranges, with live digital zooming capabilities. Specialized models include Ceta-S and Ceta-F for low-dose imaging and Ceta-D for MicroED applications.

High Quality Images

Achieve high-contrast, superior quality TEM and STEM imaging through simultaneous multi-signal detection, utilizing up to four-channel integration in STEM detectors.

Chemical Composition Data

Chemical information obtained through flexible energy-dispersive X-Ray spectroscopy (EDS) analysis.

Improved Productivity and Reproducibility

Ultra-stable column, SmartCam remote operation, and constant power objective lenses for quick mode and high-tension switches. Fast, easy switching for multi-user environments.

Automated Data Collection

Utilize Thermo Scientific Tomo 5 software for tomography and Thermo Scientific EPU software for automatically imaging single-particle analysis.

Collect large area 2D images at different image resolutions using Thermo Scientific Maps Software with automatic image acquisition and stitching while documenting the overall context and entire area of the region of interest with exceptional quality.

Compact Design

Thanks to its smaller footprint and dimensions, this tool can fit into more challenging spaces while reducing infrastructure and support costs.

Specifications

Source: Thermo Fisher Scientific – Electron Microscopy Solutions